Sentence examples for using the primary antibody from inspiring English sources

The samples were stained for the presence of basal cells using the primary antibody rabbit polyclonal anti-human cytokeratin 5 (1/50; green channel, Thermo Scientific, Rockford, IL) and ciliated cells using the primary antibody mouse monoclonal anti-human β-tubulin IV (1/2000; red channel, Biogenex, San Ramon, CA) incubated at 4°C overnight.

After SDS-PAGE, the proteins were transferred to Immobilon-P PVDF 0.45 µm membrane (Millipore, MA) and probed for the epitope FLAG (DYKDDDK) tag on all Y1 constructs using the primary antibody anti-FLAG® M2 (Stratagene, CA).

The samples were stained for the presence of ciliated cells using the primary antibody mouse monoclonal anti-human β-tubulin IV (1/2000; red channel, Biogenex, San Ramon, CA) incubated at 23°C, 30 min. Isotype matched IgG (Jackson Immunoresearch Laboratories, West Grove, PA) was the negative control.

The purified proteins were analyzed using SDS-PAGE and Western blot using the primary antibody Mouse-anti-His mAb.

Dilution experiments using the primary antibody to assess specificity and observe extinction of signal were also performed.

Immunohistochemical analysis was performed using the primary antibody for sclerostin (rabbit polyclonal, 1 200, Santa Cruz Biotechnology, USA, sc-130258).

Western blots were performed using the primary antibodies mentioned above, at a 1∶1000 dilution.

Blots were probed using the primary antibodies.

Western blot analyses were performed using the primary antibodies described in Supplementary Materials and Methods.

Immunohistochemistry was performed using the primary antibodies against GLAST (1 : 1000) and βIII tubulin (1 : 1000).

Slides were incubated for 32 minutes at 37°C using the primary antibodies listed above.