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TrkB activity was quantified using the previously described KIRA-ELISA assay [28] with slight modifications.
Activation of PPAR LBD was quantified using the previously described HGLN5 PPARα and PPARγ cell line [20].
VEGF released into the solution was quantified using the previously described ELISA methods.
The endogenous JA level was extracted and quantified using the previously described method [ 58].
Arm volume was quantified using the previously validated relative volume change (RVC) equation, which calculates change in volume compared to a pre-operative measurement [ 47].
Pre-PCR libraries were quantified by qPCR using a previously quantified and sequenced library in dilutions containing 100 ng, 10 ng and 1 ng as standard.
Moreover, our assay could be easily adapted in a quantitative format by using a previously quantified RNA to create a standard curve to which results from unknown samples can be compared.
Global baseline gene expression in liver on these samples was previously quantified using the Agilent 4x44 array [ 17] (see Methods for details).
The Ni tolerance of TgSATm was previously quantified using a metal growth, zone of inhibition assay, on plates, [ 19] (Fig 4A Ni).
PCSK9 and HMGCR splice variants were quantified using the assays previously described [4], [5].
Temporal oscillations in phase have been previously quantified using OCT [ 33].
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