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For the 1740 compound-patent pairs from Reaxys we retrieved all family members from the SureChEMBL database using the previously generated SCPN numbers.
MiaPaCa2 pp32-transfected (Mia.pp32) and empty vector (Mia.EV) cells were trypsinized and collected as previously described [25] and our generated do novo using the previously generated and purchased parental pancreatic cancer cell line (ATCC, Manassas, VA).
We then assessed whether the NEDD1 mutants that showed reduced binding to γ-tubulin could recruit γ-tubulin to the centrosome using the previously generated GFP-tagged NEDD1 CTD mutant proteins.
The resulting genes were refined using the previously generated present and absent calls.
After adjustment, the tumors were reclustered using the previously generated 5,000 lists of 70-gene lists and reanalyzed.
Here, we analyzed the tandem-repeat pattern and studied spontaneous mutation rates for microsatellite markers by using the previously generated mutation accumulation lines.
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For each step, we used the previously generated consensus sequence for the next mapping method.
This system is markedly different from the non-replicative plasmid integration-based approach used for the previously generated buk1 and pta mutants of C. acetobutylicum (Green et al. 1996).
The first one consists of a direct method, based on the simulation of each ULCF specimen under monotonic conditions and in the other one is based on the use of the previously generated 3D monotonic ductile fracture locus.
Using a modified set of the previously generated exposure estimates, we examined the exposure error and between-pollutant relationships and quantified potential attenuation of model coefficients in single- and bipollutant models at the ZIP code level for ambient-generated PM2.5, EC, SO4, CO, NOx, and O3 in Atlanta.
Using this RFP-marked insertion and the previously generated attP-FRT insertion to the left of the histone locus, we succeeded in deleting the entire 125-kb histone locus using FLP-mediated recombination (data not shown).
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using the previously negotiated
using the previously fabricated
using the previously decoded
using the previously validated
using the previously described
using the previously computed
using the previously built
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