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Compared to PP2 cells, the D17 cells generated using the previous protocol show limited GLIS3 expression, which explains why our previous studies did not detect defects of GLIS3−/− hESCs20 (Supplementary Fig. 1e).
We evaluated the DHT for line detection using the previous protocol on the House image with its ground truth (see Fig. 10).
3,4-Diphenylisoxazolin-5-one (Section 4.2.4) (0.406 g, 1.71 mmol), using the previous protocol, afforded the title compound (8d) (256 mg, 85%) as a white solid: mp 105 106°C (Et2O / n-hexane).
There is also a slight increase in the ratio of genes with published nephron-specific expression patterns (56%) compared to the gene list generated using the previous protocol (44%).
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For the purpose of testing this hypothesis we developed a weak training protocol, where the T groups received one 30 min training trial with a smaller quantity of food than that used in the previous protocols (i.e., less than 80 mg).
In contrast, the previous protocol used for pre-operative pupil dilatation at the district general hospital where the study was conducted required at least four instillations of mydriatic eye drops.
We first confirmed that this protocol also induces a significant inhibition of primary Ewing's sarcoma progression in bone at day 29 (p < 0.05, Figure 3A) as it has been published with the previous protocol used (100 μg/kg, twice a week, [ 18]).
Hemocompatibility of different samples were analyzed using a previous protocol with some modification (Katanasaka et al. 2008).
The dorsal epithelium of rodent tongue, containing both anterior and posterior taste fields, was isolated using a previous protocol [64].
The amount of nitrate and nitrite in the culture medium was measured after reduction of nitrate to nitrite, and quantification of nitrite using a previous protocol.
A peritoneal lavage (PL) was performed in the donor mice, and the harvested cells were stained with PKH-26 PCL using the protocol according the previous item.
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