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This indicates low retention in the memory of the CS-US association following conditioning using the present protocol.
In fact, using the present protocol in earlier experiments, we have reliably observed nearly 100% of females mating within the first hour of being combined with males.
The finding of similar fear conditioning in HAB and NAB mice using the present protocol suggests that selection for high trait anxiety does not necessarily result in altered CS-US association in HAB mice.
Using the present protocol, thousands of transgenic Impatiens could be produced within 3 to 4 months.
Using the present protocol of mild exercise training an increased expression of caveolin 3 at transcriptomic and proteomic levels was demonstrated.
Cells isolated from the bone marrow of cynomolgus macaques using the present protocol showed a distinct colony-forming property that was compatible with the characteristics of MSCs.
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Thus, to produce incomplete burst fractures, only one single compression was sufficient using the presented protocol.
Using the presented protocol, V5/anti-V5 and HA/HA11 tag/antibody combinations provided the most sensitive detection in brain tissue.
Using the presented protocol, V5/anti-V5 and HA/HA11 are the preferable tag/antibody combinations for immunohistochemical analysis in brain tissue while detection of myc-tagged proteins by polyclonal anti-myc antibody is not an ideal candidate.
Therefore, using the presented protocol, our findings show that all tag/antibody combinations, with the exception of myc/polyclonal anti-myc, show sufficient sensitivity and specificity for immunohistochemical analysis in brain tissue.
Conversely, PAI-1-null mice on a C57BL/6 background are resistant to diet-induced obesity (24), and this was confirmed using the present experimental protocol (Supplemental Figure 4).
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