Using the predesigned, pretested interview schedule and self-reporting questionnaire, all relevant information was collected.
SNPs were genotyped using the predesigned TaqMan SNP Genotyping Assays Applied Biosystemss, Foster City, CA, USA), C_1971047_1 and C_27464534_20, resp).
Endogenous LAMR expression was inhibited using the predesigned siGENOME individual oligonucleotide no. 3 targeting LAMR (Dharmacon, Lafayette, CO, USA).
Using the predesigned and pretested interview schedule, all relevant information regarding sociodemographic characteristics like age, education, occupation, income, addiction, and so forth, was collected.
For siRNA transfection, we used the predesigned silencer Neu-1 siRNA from Ambion (5'-GGCGAGGAAAAUGUCCUCATT-3' (forward), 5'-UGAGGACAUUUUCCUCGCCTC-3' (reverse); ID: 8481, distributed by Applied Biosystems, Courtaboeuf, France) as they are very efficient Neu-1 siRNA [9].
The energy and exergy flows between the components are calculated using the predesign tool for an optimized building design.
Bcl-xL expression was transiently downregulated using the following predesigned duplex siRNA directed against Bcl-xL (siBcl-xL; Ambion, Carlsbad, CA, USA).
Each new brick was made by hand using the specifications of the originals.
The rs7833904 polymorphism of the CYP7A1 gene was genotyped using the TaqMan Predesigned SNP Genotyping Assay (Applied Biosystems, Foster City, USA).
We used the Applied Biosystems predesigned TaqMan assays (Applied Biosystems, Carlsbad, CA, USA) for rs662 (192Q/R) and rs705379 (–108C/T) with 15 ng of genomic DNA.
