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Similar approaches can be used to study the power of association studies using the pooling design.
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However, to our knowledge, no previous studies have explored the consequences of using the pooled-islet design.
All samples in the cohort underwent Sanger sequencing of the coding regions of GATA5 as previously reported by our group, used here as a test of the pooling design as well as the sensitivity and specificity of the variant calling algorithm.
The pooling design is illustrated in Fig. 1.
We study the pooling designs constructed from affine geometries.
The idea of using elaborate pooling designs to reduce the costs of genetic screens and rare variant genotyping is appealing and useful.
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Such association tests could also be implemented by selective DNA pooling, perhaps using the new fractioned pool designs [ 29] to increase power and accuracy.
In addition, Lesch and colleagues used a pooling design, which reduces power by about 70% and puts limitations to the genetic models that can be investigated (Lesch et al., 2008).
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