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gambiae was investigated using the physical map developed in this study.
Using the physical map, we identified the BAC/BIBAC contigs containing or closely linked to two QTL, one controlling RDR and the other controlling DTF.
Physical maps of 1 1 orthologs (at subregion resolution) were constructed using the physical map of Ae. aegypti and the genome map of An. gambiae.
Using the physical map, we observed that, similar to MIB duplicons, highly divergent Mamu- B genes can be present on the same haplotype.
Out of the 219 gene loci orthologous to barley chromosome 3 H genes, 153 have been located on wheat BACs assigned to one of the eight deletion bins of wheat chromosome 3B using the physical map data [ 17].
The first BAC contig sequencing results obtained on chromosome 3B [ 28] using the physical map established with the SNaPshot technology [ 23] suggested that about 10% of the BACs were mis-assembled (unpublished data) and this was confirmed in our study (estimated mis-assembled BACs in the SNaPshot map: 9.5%).
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A synteny search was conducted using the physical maps of human, mouse and chicken genomes to determine whether the genes encoding the HR domain in human and mouse were conserved among the same set of neighboring genes.
We used the physical map developed by this study to compare genomic features, such as genes, satellites and TEs, in individual chromosomes of Ae. aegypti.
After identification of BACs carrying barley orthologs, we used the physical map [ 17] to locate them in their respective contig and possibly in one of the eight chromosome 3B deletion bins used for this study.
The integration of all the QTL on the integrated map makes it possible to more precisely compare the location of QTL mapped in different populations and to use the physical map locations of the markers to identify candidate genes for specific traits.
It is pertinent to take into account the robustness of the integrated map obtained because the same genotype used for the physical map construction was one of the parents of the mapping populations used to place BES-SSR markers.
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