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Dendograms were drawn using the PHYLIP package [23].
All phylogenetic analysis was carried out using the PHYLIP package, as implemented in the MOBYLE Portal (http://mobyle.pasteur.fr/) [23].
We first aligned the sequences using ClustalX [33] and generated a maximum likelihood tree using the PHYLIP package [34].
The neighbour joining tree was produced using the PHYLIP package [48] and based on the consensus of 1000 bootstrap replicates.
Using the PHYLIP package [30], a hierarchical tree-like representation of these data can be generated using the Fitch-Margoliash distance-based optimization method.
A phylogenetic tree constructed using the PHYLIP package suggests that these 14 Puf proteins can be grouped into three clusters: (1) the Puf-A homolog cluster, (2) the C14orf21 homolog cluster, and (3) the PUM1/PUM2 homolog cluster (Fig. 2B).
Similar(28)
The second approach used the phylip package [ 28] to apply a distance-based bootstrap analysis with 1000 replicates to each of the curated alignments.
We used the Phylip package V3.69 [ 66] to build the maximum likelihood (ML) tree for each protein family under the JTT substitution model.
Bootstrap values were obtained for branching patterns using the Phylip software package (version 3.65 [103]) and values ≥50% were included for main nodes of the tree.
Additional phylogenetic analyses were performed using the PHYLIP software package (version 3.5c; Felsenstein 1993).
The neighbor-joining (NJ) method was performed with JTT amino acid substitution matrix using the Phylip software package [ 79].
More suggestions(12)
using the phylip version
using the phylip neighbour
using the Cytoscape package
using the phylip application
using the phylip program
using the Limma package
using the TargetSearch package
using the Python package
using the phylip computer
using the AnimalINLA package
using the phylip drawgram
using the SoftWorx package
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