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For this assay, virus particles were trapped from suspension using virus-specific rabbit IgG and detected using the phage displayed scFv, an anti-pVIII mouse monoclonal antibody (mAb) and a labelled anti-mouse IgG.
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We thus used the phage displayed library to select for binders against five different antigens using purified proteins adsorbed on microtiter plates.
Our research was directed to localize functional sites in both proteins using the phage display method.
The properties of M-13 bacteriophage can be systemically modified to impart target-specific selectivity and sensitivity using the phage display technique.
We previously obtained a strong LPS-binding peptide, Li5-001, using the phage display method (Matsumoto et al., 2010. J. Microbiol. Methods. 82, 54 58).
Single-chain variable region Ab fragments were obtained from the RNA extracted from the hybridoma cells secreting mAb C7 (scFv C7) by using the phage display methodology, as described [3].
The gene population was screened for novel binding properties using the phage display method [ 23].
When using the phage display system, the cDNA of antibodies of interest becomes available without further cloning steps.
Avd mutant sbAvd-1, which was captured using the phage display selection [ 23], has a micromolar affinity to testosterone.
We successfully constructed a specific anti-FMDV type O sdAb immune library using the phage display technology.
The antibody library FAB-310 (Dyax Corp ,Cambridge, MA) was used to select recombinant monoclonal antibodies in the form of Fab fragments using the phage display technology.
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