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Our research was directed to localize functional sites in both proteins using the phage display method.
We previously obtained a strong LPS-binding peptide, Li5-001, using the phage display method (Matsumoto et al., 2010. J. Microbiol. Methods. 82, 54 58).
The properties of M-13 bacteriophage can be systemically modified to impart target-specific selectivity and sensitivity using the phage display technique.
Single-chain variable region Ab fragments were obtained from the RNA extracted from the hybridoma cells secreting mAb C7 (scFv C7) by using the phage display methodology, as described [3].
The gene population was screened for novel binding properties using the phage display method [ 23].
Avd mutant sbAvd-1, which was captured using the phage display selection [ 23], has a micromolar affinity to testosterone.
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For this assay, virus particles were trapped from suspension using virus-specific rabbit IgG and detected using the phage displayed scFv, an anti-pVIII mouse monoclonal antibody (mAb) and a labelled anti-mouse IgG.
In the current study, we used the phage display technique to generate mimotopes that complemented the screening Avastin antibody (Ab).
Using the phage-display technology, we previously selected and characterised human recombinant anti-TPO aAbs (Chapal et al, 2000, 2001).
Additionally, we investigated the in vitro and in vivo impacts of a newly established anti-human TFRC monoclonal antibody, which was isolated from a human cDNA library using the phage-display method, on cell proliferation and survival.
We previously established a procedure for the comprehensive identification of tumor-specific antibodies via the extensive isolation of human monoclonal antibodies (mAbs) using the phage-display method 12. Several antibody libraries were screened, and a large number of human mAbs that bound to the surfaces of tumor cells were isolated.
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