Sentence examples for using the pfaffl equation from inspiring English sources

Relative expression to a calibrator sample was calculated using the Pfaffl equation (Pfaffl, 2001).

Relative values of transcripts were calculated using the Pfaffl equation [ 57].

Relative expression compared to one C17.2 cDNA sample was calculated using the Pfaffl equation (Pfaffl, 2001).

Fold change was calculated as clodrolip treated versus control tumors using the Pfaffl equation [ 45].

To compute expression using the Pfaffl equation, Cq values and PCR efficiencies are required for the gene-of-interest and reference miRNA assays.

The relative expression of a defined gene (ratio between either heterozygous versus wild-type or homozygous versus wild-type of templates from the same litter) was calculated using the Pfaffl equation (Pfaffl et al., 2001).

Efficiency of individual PCR reactions was determined with the LinRegPCR tool [ 13], and efficiency of all reactions with the same target amplicon was averaged as in the (PavrgE Ct model [ 14] and used in the Pfaffl equation [ 15] to obtain efficiency-corrected, normalized relative quantification values.

Cycling parameters were determined and analyzed using the Pfaffl modification of ΔΔCt equation with taking account to the efficiency of the reaction [ 8, 9].

The relative quantification was made using the Pfaffl modification of the ΔΔCt equation, taking into account the efficiencies of individual genes.

Cycling parameters were determined and analyzed using the Pfaffl modification of the ΔΔCt equation with taking accounts to the efficiency of the reaction [ 25].

PCR reaction efficiencies were determined empirically by amplification curve analysis using LinRegPCR58 and expression ratios calculated using the Pfaffl method59 as described in detail previously60.