Sentence examples for using the peptide microarray from inspiring English sources

Current mass spectrometry techniques and novel proteomics approaches like antibody microarrays determine protein phosphorylation levels rather than the enzymatic activity resulting from it, while measurement of kinase activity using the peptide microarray provides a direct view on the extent of enzymatic activity leading to specific signal transduction.

After having established a quantitative protocol for the detection of cytolytic activity using the peptide microarrays, finally, we were interested to simultaneously detect different CTL activities and evaluate the detection limit.

Serum samples from 374 early arthritis patients were analyzed with iSPR using the synthetic peptide microarrays described above.

Incubation chambers corresponding to the size of the peptide microarray were created by using commercially available self-adhesive frames that are typically employed for the generation of incubation chambers for the hybridization of oligonucleotides (Fig.  1).

Finally, the polyanion peptide conjugates were screened for their capacity to bind selectively a recombinant MET-Fc chimera using a peptide microarray format.

Briefly, interactions were assessed using a peptide microarray followed by confirmation of positives and measurement of binding affinities by fluorescence polarization (FP), a high-quality affinity measurement method (Stiffler et al., 2007).

Currently, we foresee the major application of the peptide microarrays in epitope identification.

For example, the tyrosine phosphopeptide binding of SH2 domains have been elucidated using a quantitative peptide microarray based approach [ 18] and by the use of a high-density peptide chip technology [ 21].

We analysed the gene expression profiles in endothelial cells stimulated with antibodies affinity-purified against either the UL122 or the US28 peptides using the microarray technology.

This database contains microarray data of 40 different experimental conditions obtained through microarray analysis using the same custom microarray chip as described in this manuscript.

Using peptide microarrays, the presence of natural antibodies against Aβ in plasma samples and cerebrospinal fluid of AD patients and healthy controls aged 21 89 years was reported [ 9].