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Using the peptide conservation analysis tool embedded in HPVdB, we answered the question how many HPV strains contain this epitope.
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The rabbit phosphorylated ERK antibody was generated using the peptide GIL pT E pY VATR21 and affinity purified (BioGenes).
In Section 4, conservation laws are presented using the new conservation law theorem.
The level of evolutionary conservation was estimated using the conservation index (CI).
The antimicrobial peptides were predicted by BLAST+ using the antimicrobial peptide database (APD3).
Conservation of proteins was determined using the longest peptide associated with each human ECM gene (termed the "reference").
The conservation profile of non-exonic reads was assessed using the UCSC PhastCons17way conservation score.
Lungu, O. I. et al. Designing photoswitchable peptides using the AsLOV2 domain.
To examine whether recognition prevalence was related to the degree of peptide conservation, Shannon entropy was used as a measure of HIV-1 peptide variability or diversity (see Experimental Procedures).
Amino acid conservation at every position of the peptide sequence alignment was plotted using the "sequence logo" method [79], by pasting the alignment at the logo website (http://weblogo.berkeley.edu/logo.cgi, [80]).
Peptide similarities/identities were determined using the GeneDoc programme [ 90] and amino acid consensus sequences within each peptide group were deduced and compared to demonstrate general levels of conservation for each subfamily.
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