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Pathways and interaction maps were constructed using the path designer function, with expression changes overlayed onto the pathways.
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Using the path you have drawn, right-click over the path layer on your paths window.
Next, the PI3K/Akt and PTEN pathways were modified using Path Designer, IPA, and were further customized by adding ANXA7 versus p53 connections (genes as nodes and relationships as edges) from the current report.
The constructs were designed using the Gene Designer software and the CLC Main workbench.
PCR primers were designed using the Beacon Designer 7.0 software (Premier Biosoft International, Palo Alto, CA, USA).
Briefly, pairs of PCR primers were designed using the Beacon Designer software (version 2, California, USA).
All primers (Additional file 2) were designed using the Beacon Designer program (Premier Biosoft, Palo Alto, CA).
Gene specific primers for candidate genes were designed using the beacon designer™.
Gene-specific primers for the identified genes were designed using the Beacon designer program.
Primers were designed using the Beacons Designer software (http://www.premierbiosoft.com/molecular_beacons/index.html).html
Candidate sequences were generated using the program Gene Designer [ 42].
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