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Rather than using the parsimony procedure with 2-break operations, we study the parsimony procedure with respect to the change of half-breakpoint's states for each vertex in the breakpoint graph.
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Equally weighted heuristic tree searches were performed using the parsimony ratchet procedure [72] with 1000 replicates as implemented in the program PAUPRat on the CIPRES cluster.
Large protein datasets are similarly addressed using the parsimony and distance methods.
Phylogenetic relationships among the ciprofloxacin sensitive and resistant strains based on 9 housekeeping genes were estimated using the statistical parsimony procedure and graphically depicted as a network of gene genealogies.
LBCA gene set was determined using an empirical parsimony procedure based on COG phyletic patterns (See Additional file 1: "Reconstruction of the gene set of the Last Bacterial Common Ancestor") which assigned a COG to LBCA if it was present in several diverse bacterial clades.
To evaluate the variability of each locus, we calculated the number of characters that were constant, parsimony informative, and autapomorphic using the default parsimony settings in PAUP* [ 65].
TCS estimates phylogenetics networks from DNA sequences or nucleotide distances using the statistical parsimony algorithm which estimates the 95% parsimony limits and outgroup weights (Templeton et al., 1992).
Phylogenetic analyses were conducted on the 11 sub-alignments using the maximum parsimony criterion in PAUP* (Phylogenetic Analysis Using Parsimony *and other methods) v.4.0 β10 [ 30].
The phylogeny was inferred using the Maximum Parsimony method [42].
The evolutionary history of the yqi gene was inferred using the Maximum Parsimony method.
Shorter sequences were added to maximum-likelihood trees using the ARB parsimony insertion tool [69].
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