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Finally, unc-4 GFP unc-4 GFPned with PI were gated (Fig 2B) using the parameters established above.
Classification of subtelomeric TS sequences into the groups of the TS superfamily [ 2, 34] was carried out with BLASTx and BLASTp using the parameters established by [ 36].
Using the parameters established with the model building data set, agreement (or not agreement) to a perceived impact subscale was predicted for the testing data set.
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For this, tests 1 and 2 were applied on 100 alignments generated with Evolver under the null model of the test (M1a and modified branch-site model A with ω 2 = 1) using the global parameters established for the real dataset (branch A, B, or C).
The tests on the independent test datasets was performed using the model trained on the corresponding training dataset using the parameters and feature sets that were established based on the cross-validation.
Remaining sequences were clustered using d2_cluster [ 81] and CAP3 algorithms [ 82] using default parameters established for ESTAP.
After generating the negative controls, the RNA contigs were searched using the established parameters from control runs (see Methods).
Using synaptic vesicle parameters established by Takamori et al. (2006), we calculate that a spontaneous release rate of approx. One vesicle per second (Wyllie et al., 1994) and a neurite diameter of 440 nm (Takamori et al., 2006) can account for 0.12 μm of neurite extension per minute in lipids alone.
Semi-quantitative analysis of the TOF values of individual iron centers was done using the recently established Mössbauer parameters of the Fe(II) parameters for individual iron cationic species, i.e. β (β-1 and β-2) and α sites [1].
It is essential to understand whether the dose received in the areas of high level natural background radiation can induce detectable level of biological effects using the established cytogenetic parameters like dicentrics, micronuclei etc.
Baseline input parameters of the model were based on an "average" mammalian cell using the established, generic physiological parameters of cells (such as membrane surface area, cellular and organelle volumes, pH values in each compartment, membrane potentials, and lipid fractions in each compartment) [33, 39].
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