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A particularly useful way of capturing epidemic dynamics on networks is by using the pairwise model (Keeling 1999).
The Ka, Ks, and Ka/Ks values of the target FBX gene was obtained by comparing its coding sequence to that of the MRCA using the pairwise model of CodeML (PAML4 package [66]).
The winning model M5 is then further tested using the pairwise model comparison scheme as previously.
For each metabolite, open diamonds indicate the center of phenotype estimated using the pairwise model while closed diamonds indicate the estimated location using the three-way model.
Similarities between response pairs calculated using the conditionally independent model were binned (x-axis) and the mean and standard deviation of the similarity for the same pairs was calculated using the pairwise model (y-axis).
The measure of synonymous sequence divergence in coding regions (K S ) for gene paralogs was recalculated using the pairwise model (runmode = −2) of the codeml program in the PAML package [ 62].
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Dotted lines show estimated variance using solely the main effect loci without interactions (additive model), solid lines show the distribution of estimated variance across the metabolites using the pairwise epistasis model while the dashed lines are the results for the three-way epistasis model including the most prevalent three-way interactions as indicated by the epistatic network.
For each metabolite, open diamonds indicate the center of phenotype estimated using the pairwise epistasis model while closed diamonds indicate the estimated location using the three-way epistasis model.
To test directly for epistatic interactions between the detected QTLs, we conducted an ANOVA using the pairwise epistasis model.
Using the pairwise epistasis model analysis separately for all metabolites, we identified all significant main effect markers, individual genetic loci, and interactions, epistatic terms.
Phylogenetic trees were constructed using the neighbor-joining method with genetic distances computed using the pairwise deletion model and bootstrap analysis of 500 values and root on midpoint (i.e. midpoint of the longest pathway between two clusters of sequences).
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using the pairwise similarity
using the optimization model
using the pairwise key
using the fitness model
using the business model
using the pairwise haplotype
using the pairwise gossip
using the pairwise complete-linkage
using the agency model
using the pairwise homoplasy
using the pairwise alignment
using the admixture model
using the pairwise approach
using the studio model
using the pairwise kinship
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