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The divergence between each LTRr copy and the corresponding consensus was estimated as the proportion of nucleotide differences with the aid of MEGA version 2.1 [45], using the pairwise deletion option.
Amino acid identity was calculated with MEGA 4 using the pairwise deletion option.
All the deletions and gaps arising from the alignment were eliminated by using the pairwise deletion option.
The Neighbor-Joining (NJ) tree was constructed in MEGA version 4.0 (Tamura et al. 2007) using the pairwise deletion option.
We used MEGA3 [ 50] to infer the NJ phylogenetic tree, using the pairwise deletion option and Poisson correction distance.
DNA alignments were used to estimate pairwise distances and to build phenetic trees, using the pairwise deletion of gaps option of the MEGA4.1 software [ 69].
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When we used the pairwise deletion option for neighbour joining (NJ) in MEGA4 we obtained the same tree topology.
First, we checked the boundaries of the different families through the construction of a sequence identity matrix using the pairwise gap deletion method (see Additional file 1).
A neighbor-joining (NJ) phylogenetic tree was constructed using Mega5 with the pairwise deletion parameter selected for gaps/missing data as well as the nucleotide substitution model parameter selected for the Maximum Composite Likelihood Model.
Identity values between the test sequences and the references were calculated using the MEGA5 program with the pairwise deletion option.
The NJ tree was constructed using MEGA [ 55] with the "pairwise deletion" option and "Poisson correction" model.
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