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Glutamate decarboxylase activity was assessed using the pH indicator dye bromocresol green; the dye changes from yellow to blue upon pH increase in the reaction mixture, following decarboxylation of L-glutamate (Fig. 2A).
Protons released from the hydrolysis reaction were measured using the pH indicator cresol purple.
The net production of protons was measured using the pH indicator cresol purple.
Protons released during the hydrolysis of lactones were measured using the pH indicator bromothymol blue.
In addition to providing quantitative information about intestinal emptying rate, we were able to extract further information about the nature of Drosophila excreta by using the pH indicator dye Bromophenol blue (BPB, Figures 2E 2G) to obtain colored deposits, which we then analyzed semiautomatedly to extract quantitative information about size, shape, concentration, and color.
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pHi of saponin-isolated parasites was measured using the pH-sensitive indicator BCECF (Molecular Probes, Invitrogen) as described previously (Saliba and Kirk, 1999).
Bromocresol purple (0.006%) was used as the pH indicator.
The pH indicator strips used had a possible range of 0-6.0 0-6.0
The pH indicator dye Cresol Red was used to detect pH changes in the bulk aqueous phase.
We were able to reproducibly screen for TE activity using standard MacConkey agar with lactose as the sugar source; the pH indicator neutral red changes from red (pH 6.8) to yellow (pH 8.0).
The pH indicator showed good sensitivity around physiological pH, making it potential useful as a simple visualization pH indicator from detection of intracellular micro-environment.
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