Sentence examples for using the outer amplification from inspiring English sources

PCR products were purified with the QIAquick PCR purification kit (QIAGEN, UK), and sequenced using the outer amplification primers and several internal sequencing primers, using BIG DYE terminator v3.1 chemistry (Applied Biosystems, UK) and an ABI 3130xl capillary sequencer (Applied Biosystems, UK).

Amplification of the RLEP regions in M. leprae was performed both as standard PCR and as a nested PCR [24] with amplification of a 129 bp product using the outer primers LP1 and LP2 (Table 1) which can be nested to a 99 bp product in the second round of amplification using LP3 and LP4 (Table 1).

The first round involved the amplification of a 600 bp fragment using the outer primers HSV-N1-For (5'-GGGTTCCCACCAACGTCTCC) and HSV-N1-Rev (5'-GGGTGTGTGCGTCGCCCGC).

As PCR assay was concerned, the amplification was carried out in 50 μl reactant, using the outer primers F3 and B3.

Then, two PCR amplifications are preformed per library: the first uses the outer adaptor primer (AP1, provided by the manufacturer) provided in the kit and the outer, gene-specific primer (GWRUT C30gsp1; Table 1).

Some operators prefer not to use the outer sheath.

Two microliters (2 μl) of the sample DNA were used for outer amplification.

Amplifications were performed using the original amplification primers.

RNA amplification of 400 ng total RNA was performed using the Illumina TotalPrep Amplification Kit (Ambion), according to manufacturer's instructions.

using the amplification primers.

Initial PCR was carried out using the 5′ RACE outer primer and gene-specific outer primer.