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The corresponding cAMP concentration was 8.50 g/L, which was about 1.8-fold increase compared with that using the original medium.
A maximum PCA yield of 1240 mg/l was obtained at 17.81 g/l glucose and 11.47 g/l soytone, and the production was increased by 65.3% compared with that using the original medium, which was at 750 mg/l.
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To investigate the effects of nitrogen deficiency on biomass and lipid yield, N. salina was batch cultured autotrophically and axenically in N-deficient conditions using the original source of nitrogen in f/2 medium (sodium nitrate).
Yeah, instead of using the original score.
This time, using the original feather color.
In that latter case, LOFLO nutritive medium (Formedium) was used because of a reduced autofluorescence compared to the original medium.
The optimized medium provided a 93% increase in final vitamin concentration compared to the original medium.
l-lysine production with the optimised medium increased 2.6 times as compared with the original medium.
The original medium was replaced with serum-free fresh ESC medium 12 h later.
The enzyme production increased three fold compared to the original medium.
Figure 2 shows accumulation of monacolin K content from Monascus M1 in the original medium and glutamic acid-containing medium.
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