Sentence examples for using the original amplification from inspiring English sources

Amplifications were performed using the original amplification primers.

We sequenced both strands of these amplification products using the original amplification primers and a suite of internal primers.

We sequenced both strands of the PCR product using the original amplification primers and multiple internal primers.

PCR products were then cycle sequenced using the original amplification primers and either the CEQ Dye Terminator Cycle Sequencing Quick Start Kit (Beckman Coulter, Fullerton, CA) or BigDye Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, CA).

Following the PCR amplification using the original primers and PCR conditions, the products were ligated into pGEMT-Easy vector (Promega; purchased from VWR Canlab, Mississauga, ON, Canada) and cloned using Top 10 Competent cells (Amersham Biosciences).

A volume of 3 to 5 μl of the supernatant was used for re-amplification using the original PCR conditions and primer pair without a GC clamp and then photographed using a G BoxiChemi 2D gel image analyzer using Genesnap software 7.03 (SYNGENE, Synoptics, Ltd, Cambridge, UK).

E2, E6 and HMBS amplicons were cloned separately, following PCR amplification, into pcr®2.1 vector using the original TA cloning system (Invitrogen).

Following PCR amplification, the real-time PCR amplification products for erbB2 and β-actin were cloned separately into the pcr 2.1 vector, using the original TA cloning system (Invitrogen).

Second, we failed to obtain the anomalous mtDNA type in some cells when using the original cell lysate as the template in an independent assay, which suggested that it was most likely introduced during the amplification process.

This time, using the original feather color.

For subsequent fragmentation using the Affymetrix protocol the original amplification protocol was modified by adding 2.3 µl of 10 mM dUTPs in the PCR master mix (total volume per reaction: 61 µl).