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The composition and content of the secondary structure of the protein were fitted using the Origin program, and the CDpro software was used to fit the protein (BSA) secondary structure.
Raman spectra of selected minerals were processed using the Origin program and Lorentzian peak fitting was used to provide a more accurate estimation of the height, position and width of the Raman peaks of the selected minerals for this study.
The corresponding standard curves (Figure 1) were constructed by plotting the average relative absorbance against the SSd concentration, with three replicates per concentration, using the Origin Program 6.0 software package.
The monomer conversion, as a function of reaction time, was determined by integration of the reaction heat curves using the Origin program.
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Simple linear regression analysis was applied using the Origin 6.0 software program.
All calculations and construction of calibration curves were performed using the Origin (Microcal Software Inc., Northampton, MA, USA) and Excel (Microsoft Corporation, Redmond, WA, USA) programs.
Data were analyzed using the Origin software.
The association rate constant (ka) for subunit association in the stopped-flow light scattering experiments was estimated by non-linear regression (22) or by the Origin Program, using the three-parameter relation Eq. [10].
The precision of the method was calculated by comparing the determined quantity of the substance with the quantity of substance calculated based on statistical analysis using the program ORIGIN 7 (Standard/Professional versions).
Diffusion maps were generated by interpolation from the FCS measurement points using the program Origin (OriginLab Corporation, Northampton, MA, USA).
The titration data were analyzed using the program Origin 7.0 and fitted by the one-site binding model.
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