Sentence examples for using the optimized protocol from inspiring English sources
Using the optimized protocol deduced from the results of the orthogonal experiments, the electrotransformation efficiency of Dietzia sp. DQ12-45-1b reached 2.51 × 107 CFU μg− 1 plasmid.
T cells modified with the ΔCD34-tk gene using the optimized protocol should improve the overall efficacy of the HSV-tk/GCV suicide gene therapy method of GVHD control.
The EM microbiological activity was determined with the fluorescein diacetate enzymatic hydrolysis (FDA) method, using the optimized protocol for soil samples developed by Green et al. [13]; the microbiological activity was expressed in terms of the amount of fluorescein (mg/kg of EM/h) produced through FDA enzymatic hydrolysis.
They were transfected using Lipofectamine RNAi-max (Invitrogen) using the optimized protocol provided by the manufacturer for HEK293 and HeLa cells.
The scrambled control sequence used was r AAU UCU CCG AAC GUG UCA CG dT Duplexes were transfected using Lipofectamine RNAi-max (Invitrogen) using the optimized protocol provided by the manufacturer for NIH3T3 cells.
Fc2 (4 μg) was incubated with no FGE, FGE, or heat-killed FGE using the optimized protocol previously described.
To measure the accuracy of our UDPS protocol, the SG3Δenv plasmid was diluted to 1 copy, amplified using the optimized protocols described above and subjected to UDPS.
We followed the standard protocol according to the manufacturer's instructions for BL and SCL samples, but used the optimized protocol for FFPE samples.
This improved performance of Vina holds for binding sites predicted by eFindSite as well, where using the optimized docking protocol improves RMSD by 2.5 Å and increases the fraction of binding residues and specific contacts by 10 % on average.
Using the optimized experimental protocol complemented with the microarray analysis methodology, as presented in this study, we identify 49 genes as DE between the MNs and dCINs.
Labelled targets from two cell lines, Cal51 and ZR-75-1-1, were generated using the optimized purification protocol.
