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This may be compared to PYT activity of 160 IU/mL obtained using the optimized media with 1% GrCf and suggests that it optimally provides the requirements of phosphate.
Predicted and experimental dry cell weights obtained using the optimized media were 2.046 and 2.54 g/l indicating 97% agreement.
The ultimate biomass concentration of E. faecalis CGMCC1.101 using the optimized media reached 3.5×109 CFU/ml.
The effect of varying the phosphate concentration by addition of sodium phytate and KH2PO4 using the optimized media formulation with 1% GrCf was thus studied.
Using the optimized media, the production was successfully scaled-up further and improved up to 164 IU/mL in 96 h by studies carried out employing 2 and 10-L fermenters.
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Second, the adult medium Tc concentrations (1, 3, 10, 30, 100, or 300 μg/ml) were tested over three generations by using the optimized larval media concentrations (1 μg/ml for LLs #29, #66, #72, and #671010 μg/ml for LL #68) in between the adult stages.
Tests were made using the (optimized) log of SPL and a non-optimized log.
Using the optimized annotations, Kendall's W was 0.384.
The applied methodology was validated using this optimized media and enzyme activity 155.89 ± 1.68 U/l and specific activity of 0.468 ± 0.088 U/mg protein was obtained.
The optimized media formulation was studied in scaled-up larger volume batches using fermenters to ensure maintenance of the PYT production throughput.
The optimized media was further optimized for pH and nickel concentrations.
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