Sentence examples for using the optima from inspiring English sources

Fluorescence was quantified using the Optima software program on an Optima FLUOSTAR fluorimeter.

Data were analyzed using the Optima microplate reader software (BMG Labtech).

Supernatants were collected, combined, and then assayed for the presence of secreted VEGF (specific for recombinant human VEGF165 and recombinant human VEGF121) using the Optima fluorescence plate reader (BMG Labtech, Durham NC, USA).

For staining intensity detection a Zeiss Axiophot microscope coupled to a 3-chip CCD-camera was used and the analysis was performed using the Optimas 6.00 image analysis program (Imaging Technology Inc., San Diego, CA, USA).

The movement of single cells within a colony was analyzed by tracking each cells boundary, for each frame in a series, using the Optimas 6.0 motion analysis (Meyer Instruments, Houston, Texas).

After 24 h, quantification of fluorescence signals was performed using the Fluostar Optima fluorometer (BMG Labtech, Cary, NC, USA) at excitation 480 nm/emission 520 nm.

The fluorescence intensity was measured before and after injection of 1 μM NPS using the Fluostar Optima microplate reader (BMG Labtechnologies, Offenburg, Germany).

The Fluo-4 indicator was excited at 488 nm and analyzed at 518 nm using the Fluostar optima (BMG Labtech, Netherlands).

After 24 hrs, the HUVEC cells on the top of the polycarbonate membrane were removed, while the HUVEC cells attached to the bottom of the membrane were stained for 1 hr with cell viability indicator Calcein AM Fluorscent Dye and quantified using the FLUOstar OPTIMA.

The cells attached to the bottom of the membrane stained for 1 hr with cell viability indicator Calcein AM Fluorscent Dye (BD Biosciences, Franklin Lakes, NJ) and quantified using the FLUOstar OPTIMA at 495 mm excitation and 515 nm emission (BMG LABTECH Inc., Cary, NC).

These structures were then analyzed as digital images using the program Optimas 6.5.1 (BioScan Corp).