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The signal intensity was measured using the optical density at a wavelength of 450 nm (OD450).
Fig. 4 Evaluation of the cell viability by MTT assay using the optical density value.
The growth in the yeast cultures was found out using the optical density (OD600) of the culture media.
R. toruloides was cultured in the media under controlled parameters and monitored daily over a period of 5 days using the optical density of the culture.
Next, the bacterial biomass was centrifuged at 2000g for 10 min, resuspended in sterilized water, and adjusted to 109 cells mL−1 using the optical density at 496 nm.
Autoradiograms from ISH and receptor binding studies were analyzed using the optical density readings (grey intensity).
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The standard curve was made using the optical densities of known peptide concentrations, and it was used to measure the amount of target peptide in different plant lines.
To eliminate this uncertainty we used the optical density of the cultures, and the equation derived from Figure 5D, to calculate the ATP-dependent luminescence per 106 bacteria.
For each positively immunostained section, one negative control section (the next in a series of consecutive sections) was also used, and the optical density of this control section was subtracted from that of the stained section.
The protein expression levels were analyzed using a semiquantitative method of relative optical density that was calculated using the integrated optical density of the target band divided by the integrated optical density of the reference band.
GAP-43 protein expression in the sciatic nerve was also measured and processed by this method using the average optical density (mean density).
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