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A putative promoter located approximately 50 bp upstream from the TTG initiation codon of salK, was predicted using the online server of Neutral Network Promoter Prediction.
Minimal free energy mfold predictions, performed using the online server of Zuker (http://mfold.bioinfo.rpi.edu/cgi-bin/rna-form1.cgi) indicated that the most stable RNA fold was the bulged stem-loop shown in Figure 4.
It should be pointed out that, even though 4-OHT docked well to the Hsp90 ATP pocket, no similarity was identified between the structural chemistry of this site and of the ligand binding pocket of the estrogen receptor using the online server SuMo [24].
The dot plot was generated using the online server at http://www.vivo.colostate.edu/molkit/dnadot/.edu/molkit/dnadot/
Non-classical secreted putative proteins were predicted using the online server hosted by http://www.cbs.dtu.dk/services/SecretomeP/[ 21].
The protein sequences were mapped to KEGG [ 59] pathway using the online server KAAS [ 77] (http://www.genome.jp/tools/kaas/).jp/tools/kaas/
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Prediction of intron/exon splice sites was carried out using the online servers http://deepc2.psi.iastate.edu/cgi-bin/sp.cgi/ and http://www.fruitfly.org/seq_tools/splice.html.html
Probable secondary structure of gamma-kafirin protein was predicted using the online PSIPRED server.
A Roku rep told The Huffington Post that you can stream video from a PC to Roku using the online media server Plex.
Protein alignments were converted into nucleotide alignments using the online PAL2NAL server (http://www.bork.embl.de/pal2nal/) [38].de/pal2nal/
3-D structural modeling of the ZFP backbone was carried out using the online Robetta server (Washington University)[ 29, 30].
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