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He opened the first learning center in his home last summer, using the online program, as reinforcement.
The data were analysed using the online program RT2 profiler PCR array data analysis, provided by SABiosciences http://www.sabiosciences.com/pcr/arrayanalysis.php).sabiosciences.com/pcr/arrayanalysis.php
Student Leslie Calderon, 11, who had participated in the after-school class, said she felt confident using the online program and reading codes.
Hydrophilicity and hydrophobicity of the N-terminal regions was calculated using the online program http://www.innovagen.se/custom-peptide-synthesis/peptide-property-calculator/peptide-property-calculator.asp.
Using the online program DNAworks http://helixweb.nih.gov/dnaworks [48], the gene sequences of hCCR5, hCCR3, hCXCR4 and hCX3CR1 were optimized using human codon preference (Figure S1).
We designed the target DNA sequences corresponding to NPC2-shRNA using the online program (Applied Biosystems/Ambion, Austin, TX) and ligated the target DNA to the pSilencer3.1-H1 hygro vector (Applied Biosystems/Ambion).
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To assist in parsing the sequence into oligos (Figure S2), we used the online program DNAWorks (http://helixweb.nih.gov/dnaworks) developed by Hoover and Lubkowski [25].
We used the online program Fingerprint [19 - http://evol.mcmaster.ca/fingerprint] to illustrate the heterogeneity at a specific site within the barcode region as a percentage of the vertical line drawn to represent each base pair.
The ICT will use the online program TEO and mobile phone SMS.
The HIVDR mutational analysis was performed using the online "HIVdb Program: Sequence Analysis" program from Stanford University [ 11].
Sequences were analyzed using the Seqscape program (Applied Biosystems), and similarity values were determined using the online BLAST program at NCBI.
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