Sentence examples for using the oligonucleotides shown from inspiring English sources

The inversion was verified by PCR using the oligonucleotides shown in Supplementary Table S6.

Stable L6 GSK3α/β double knockdown cell lines were generated in a similar manner to that described previously using the oligonucleotides shown in Supplementary Table S1 [ 22, 23].

To determine the XPC PAT polymorphism, intron 9 of the XPC gene was amplified by polymerase chain reaction (PCR) using the oligonucleotides shown in Table 2 (primers and conditions were previously described [ 12]).

The PCR amplification was carried out with 10 min at 95°C, followed by 50 cycles of 15 s at 95°C and 1 min at 60°C, using the oligonucleotides shown in Additional file 1: Supplemental Table S1.

GFP-LIMK1/2 mutant constructs were generated by site-directed mutagenesis using the oligonucleotide pairs shown in Table 1.

Nineteen overlapping PCR products generated using the oligonucleotide primer pairs shown in Table 1 yielded a total of 15,385 bp of sequence data.

cDNAs corresponding to the EST sequences were obtained by PCR amplification, and full-length cDNAs were obtained by RACE (Cap Fishing; SeeGene, Seoul, South Korea) using the oligonucleotide sequences as shown in Additional file 13.

A knockdown of each of the miRNAs in the cluster using morpholino oligonucleotides showed a decrease in hair cell number.

The resulting cDNAs were PCR-amplified in GoTaq Green Master Mix (Promega) using oligonucleotides, showed in Additional file 4: Table S1.

Loss or gain of functions experiments using chemically synthesized oligonucleotides shows potentials of miRNAs as novel therapeutic targets.

Upon receipt of the samples at Fort Collins, a reverse transcription PCR was performed to amplify cDNAs from all 3 segments of the orthobunyavirus genome by using the consensus oligonucleotide primers shown in the Table and conditions and methods previously described (8 ).