Sentence examples for using the oligonucleotides listed from inspiring English sources

Clone inserts were sequenced using the oligonucleotides listed in the Supporting Information (PCR Primers S1).

The mutations were made using the oligonucleotides listed below (mutations in lower case and underlined) and their reverse complements using two stage PCR as previously described [17].

F5-GFP (encoded by p582-c30, GU994009) was constructed using the oligonucleotides listed in Table S2 and the Multi Quick Change Mutagenesis Kit (Stratagene).

For the construction of truncated human pfk-M genes, BamHI/XbaI fragments of nine different lengths were prepared by PCR reactions using the oligonucleotides listed in the table S1 and then sub-cloned into the pALTER-Ex1 plasmid under the control of the tac promoter.

AttL2 insertions and gene inactivation were verified using the oligonucleotides listed in Supplementary Table S6.

For all PCRs, partial but intron-spanning parts of the SEO genes were amplified using the oligonucleotides listed in Additional file 6.

The amplification protocols described in (Grimaldi et al. [2005]) were employed for PCR analysis of the aminoglycoside 3'-phosphotransferase (kanR) and sgfp genes, using the oligonucleotide primers listed below.

T82A, T82S and H192W mutant recombinant DDC plasmids were prepared using recombinant drDDC Impact™-CN plasmid as a template by a QuikChange kit (Stratagene, USA) using the oligonucleotide primers listed in Table 3. Escherichia coli DE3 competent cells were transformed with the recombinant plasmids (containing WT or a mutant DDC sequence).

For Southern blot analysis, the digoxigenin-labeled probes were generated through PCR amplification using the oligonucleotide primers listed in Additional file 1: Table S3 (PCR DIG Probe Synthesis Kit, Roche).

Pike Elovl5 cDNA was cloned via RNA extraction, cDNA synthesis and a variety of PCR techniques using the oligonucleotide primers listed in Table  2.

Plasmids were sequenced using the oligonucleotide primers listed in Table S4, and errors were verified by sequencing related plasmids for comparison.