Sentence examples for using the oligonucleotides described from inspiring English sources

The point mutations were introduced by PCR using the oligonucleotides described in Table 1.

Construction of sasF, arcA and vraS mutants was performed as described by Horsburgh et al. [72] using the oligonucleotides described in Table 14.

Genotype was determined by standard PCR, using the oligonucleotides described in Supplementary methods.

The detection of the CagA gene was carried out by PCR in the gastric mucosa of all patients, using the oligonucleotides described by Covacci et al. [ 23].

The presence of HP was detected by a commercially available rapid urease test (Promedical, Brazil), and the negative results were confirmed by PCR using the oligonucleotides described by Covacci et al. [ 23].

Capsule detection and typing of Hib were carried using the oligonucleotides described by Howie et al. [ 26]. 16S PCR detection was carried out on NP swabs that did not yield any of the pathogens assayed using the following primers: 338f-5'-ACTCCTACGGGNGGCNGCA-3' and 1046r-5'-CACGAGCTGACGACANCCATGCANCACC-3'.

a. 'RTE', ready to eat; 'CSF', cerebrospinal fluid b. '+', amplicon of expected size detected using PCR using oligonucleotides described in Additional file 6; '-', no amplicon detected c. 'FS', frameshift resulting in a truncated coding sequence encoding butyrate kinase; 'WT', wild type.

PCR amplification was performed in ChIP fragments using the oligonucleotide sequences described previously.

PCR amplification of the vacA signal sequence and mid region was performed by using the oligonucleotide primers described by Atherton et al., [ 15].

Site-directed mutagenesis (SDM) was carried out on the pEFHA wt K-Ras plasmid using the QuikChange II SDM kit (Agilent, South Queensferry, Edinburgh, UK) according to the manufacturer's instructions to generate the following K-Ras mutations: G12V, G12D, G13D, Q61H, L19F, K117N, A146T and R164Q, using the oligonucleotide primers described in Table 1.

Quantitative RT-PCR for evaluation of TNFRSF10A (DR4) and TNFRSF10B (Dreceptorstors, as well as the internal controls β-Actin, GAPDH, and cyclophilin B, was performed with the same instrument using the oligonucleotide primers described below custom synthesized at Gene Link (Hawthorne, NY, USA).