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The antibacterial assessment was performed using the nutrient agar diffusion method and measuring the inhibition zones (cm).
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A serial dilution of the wastewater samples was performed using plating onto the nutrient agar (NA) plate before and after the treatment (Alo et al. 2012).
These inocula were used for seeding of the nutrient agar.
The nutrient agar plate was used for the inoculation of both bacterial strains.
The surface of the nutrient agar was completely inoculated using a sterile swab, which was steeped in the prepared bacterial suspension.
Overnight broth culture of the bacterial isolates and fungal isolates were seeded over the nutrient agar and potato dextrose agar plates respectively using sterile cotton swab to make lawn culture.
By using diffusion method test organisms were previously seeded (10 CFU/mL) in the nutrient agar medium [ 33].
The nutrient agar, nutrient broth (NB), and Luria broth (LB) used as rich media for bacterial growth were purchased from Becton, Dickinson and Company (Franklin Lakes, NJ, USA).
The six tested organisms were swapped over the nutrient agar medium, and the disks containing silver nanoparticles were kept over the medium using sterile forceps.
The nutrient agar medium was purchased from Hi Media (Mumbai, India).
Briefly, 100 μl of the test bacteria/fungi was spread onto the nutrient agar and potato-dextrose agar plates respectively.
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