Sentence examples for using the nupage system from inspiring English sources

Depletion was confirmed by SDS-PAGE/western blotting using the NuPAGE system according to manufactures protocols (Invitrogen, Taastrup, Denmark).

Samples were separated using the NuPAGE system (Invitrogen).

SDS-PAGE was performed using the NuPAGE system (Invitrogen, Paisley, UK).

Gel electrophoresis and blotting were performed using the NuPAGE system from Invitrogen according to the manufacturer's instructions; as previously described [ 24].

SDS-PAGE was performed on 25 μg sample/lane according to Laemmli method[ 16] using the NuPAGE system (Invitrogen, Carlsbad, CA). 10 μl of Kaleidoscope protein marker (Bio-Rad, Hercules, CA) was run in at least one lane.

Samples were run on a 4 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis gel using the NuPAGE system (Life technologies) and semi-dry transferred to Imobilon-P membrane (Millipore).

After centrifugation, cleared lysates were separated by polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto polyvinylidene fluoride transfer membranes (PVDF, GE Healthcare) using the NuPage electrophoresis system (Invitrogen).

Blotting was performed on a Hybond nitrocellulose membrane (cat# RPN2020D, GE Healthcare) using the NuPAGE blotting system (Life Technologies, USA).

Cells were lysed, and proteins were analysed using the NuPAGE electrophoresis system (4 12%) (Invitrogen) and Western blotting.

Electrophoresis (SDS-PAGE) was performed using the NuPAGE Novex system with 10% Bis-Tris gels, MES-SDS running buffer, and Novex Sharp Protein Standard prestained molecular mass markers.

For western blot analysis, 30  μg of each cell lysate was separated using the NuPage electrophoresis system (12% pre-cast polyacrylamide gels, Invitrogen) and transferred to nitrocellulose membranes (Whatman, Maidstone, Kent, UK).