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Concertina was removed using the null allele [13].
However, genetic removal of sev using the null allele sevd2 [53] has no effect on R7 specification in D-cblK26 mosaic background (Fig. 6).
We ran the same analysis as above with the nine locus dataset without using the null allele model to determine if the results would change based on these two different models.
Loss-of-function crb clones, using the null allele crb11 A 22 [ 25], exhibited no apparent defects in differentiation or cell morphology (see Additional file 2, panels A-B), although during pupal development cell morphology defects become apparent within the developing photoreceptor cells [ 32, 33].
Similar(56)
In the experiments presented in this manuscript, however, we used only the null allele.
Since Stam is known to be a direct partner of Hrs within the ESCRT-0 complex [6], we tested a putative role of hrs during tracheal cell migration in the ASP using the hrsD28 null allele.
This type of genotyping failure would not be detected by the measures we used to assess the null allele classes if it produced missing values in both families.
In addition to observed heterozygosity (Ho), we calculated expected heterozygosity (He) and estimated the null allele frequency using the program CERVUS v2.0 [ 69].
We next analyzed this interaction by blocking Rasl11b function in Zoep mutant (As for the epistatic analysis described above, we used the oeptz57 null allele [23]).
In order to address the function of BALL for neuronal development, we used the ball null allele (Herzig et al., 2014).
The null allele frequencies, estimated using Brookfield's formula (Brookfield, 1996), were 0.178 (CpUZ001), 0.119 (CpUZ004), and 0.267 (CpUZ0.267
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