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A phylogenetic tree was reconstructed from the concatenated alignment using the NJ approach and the software described as earlier.
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The HA-based phylogenetic tree was constructed using the NJ method and the tree topology was further confirmed by using the Bayesian approach as implemented in Mr Bayes 3.2 [29].
Trees were generated using the NJ and ME methods.
Phylogenetic trees were reconstructed using the Neighbor-Joining (NJ) approach (Saitou and Nei 1987).
After the genetic distances were calculated for the 100 replicate data sets, phylogenetic trees were constructed using the Neighbor-Joining (NJ) approach based on the genetic distances [ 52].
The phylogenetic tree in Figure 2 was made from ClustalX alignments by MEGA2 [ 42], using the neighbour-joining (NJ) approach with complete deletion of gap positions, Poisson correction of distances and 500 bootstrap steps.
Phylogenetic reconstruction was performed using neighbor-joining (NJ) approaches with JTT and P-distance models based on full-length protein sequences, which resulted in generally similar topologies.
The final evolutionary tree of the oxygenase superfamily was computed using the fast neighbor-joining (NJ) approach [ 29] (for all sequences analyzed) and the computationally expensive maximum likelihood (ML) approach [ 30](for the selected members of each major lineage, including all hABHs).
Chipotle uses the same approach.
The PISA service uses the first approach.
Use the survey approach.
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