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As a step toward developing such a system, we have produced and tested a series of highly sensitive and specific functional gene arrays using the NimbleGen platform.
Using The NimbleGen platform with high-density expression arrays, Batista et al. [ 8] compared the transcriptomes of granulosa-like cells overexpressing, or not, FOXL2, one of the earliest ovarian markers which, together with its targets, can serve as a model to study ovarian development and function in normal and pathological conditions.
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In a genome-wide expression analysis using the NimbleGen microarray platform, of Auxin/Indole-3-Acetic Acid (Aux/IAA) and Auxin Response Factor (ARF) in Populus (Populus trichocarpa clone Nisqually-1), the genes in a subgroup of Aux/IAA showed differential expression among different tissue types [ 18].
To test whether the association of MYCN at sites of hypermethylation is correlated with reduced expression of genes, we have analyzed the Kelly and SK-N-AS cell lines using the NimbleGen expression microarray platform.
Methylated DNA immunoprecipitation followed by microarray analysis using the Nimblegen '3 × 720 K CpG Island Plus RefSeq Promoter' platform was applied in order to carry out genome-wide DNA methylation profiling covering 20 404 promoter regions.
These finished libraries were enriched using the Nimblegen SeqCap kit.
Microarray was carried out using the NimbleGen microarray service.
Images were extracted and processed using NimbleScan v2.4 software and analyzed using the NimbleGen commercial pipeline.
cDNA was labeled using the NimbleGen Dual-Color Labeling Kit (NimbleGen) according to manufacturer's protocol.
The probes were designed and printed by Nimblegen using the Nimblegen probe design pipeline previously published (Roche Nimblegen Probe design Fundaments 2008).
After incubation, arrays were washed using the NimbleGen Wash Buffer Kit and dried using the NimbleGen Slide Dryer.
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