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De novo assembly of the Roche 454 (fragment and pair-end) reads was performed using the Newbler algorithm (see Methods).
We assembled the genome of S. arboricolus using the Newbler algorithm (v2.3, Roche) for de novo assembly of reads generated by the 454 pryosequencing platform.
The first two, which were called N1 and N2, were initiated by creating a reference sequence set by using the Newbler algorithm (version 1.1.03, provided with the GS FLX sequencer) to assemble all the unmasked sequence data into contigs.
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All of the H. brasiliensis cDNA data were first filtered by quality scores, presence of adapters, PolyA/T tails and repetitive elements using preprocess.pl Perl script from est2Assembly [ 35], and then assembled into contigs using the Newbler de novo assembler algorithm of the gsassembler (Newbler version 2.7, Roche 454).
The generated reads were assembled de novo into contigs using the Newbler assembler (Roche).
The 454 data were processed using the Newbler assembler (454 Life Sciences), and the final genomic consensus sequence was obtained using the Phrap algorithm.
The sequence assembly and scaffolding was done using the Newbler assembler software v2.9.
Reads were aligned and assembled using the Newbler assembler software provided with the GS FLX instrument.
The quality filtered reads were assembled into contiguous sequences using the Newbler Assembler software (http://www.454.com/).
Assembled genomes (using the Newbler assembler) were provided by each facility.
Draft genomes were assembled using the Newbler (Roche) and Velvet [52] assemblers.
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