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HM and DH purified β-glucosidase using the new purification protocol.
The homogeneity and concentration of the core Mediator preparation were substantially improved using the new purification method, and we obtained a highly homogeneous Mediator preparation at a concentration of over 1 mg/ml.
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A batch purification assay was performed using the new detergents.
Field emission scanning electron microscopy, high resolution transmission electron microscopy, thermogravimetric analysis, X-ray diffractometry, and Raman spectroscopy were used to determine the effects of the new purification process on the SWCNTs.
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GlyR‐βL‐intein was expressed in E. coli ER2566 for 14 h at 18°C and affinity‐purified in 300 mM NaCl, 50 mM Tris pH 8.0 containing 1 mM EDTA using the IMPACT protein purification system (New England Biolabs; see Schrader et al, 2004).
The amplified cDNA was purified using the QIAGEN PCR purification kit.
RNA products were purified using the MiraColTM Purification Columns (Arcturus).
RNA products were purified using the MiraCol™ Purification Columns (Arcturus).
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