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Embryonic phenotypes were then analyzed using the neuronal marker anti-HRP and the relative amounts of Fra, Robo or RoboSD proteins were assessed using anti-HA antibodies.
Next, we performed double-labelling experiments using the neuronal marker MAP2 and the glial marker GFAP.
Protein quantification of FAIM-L using the neuronal marker NeuN for the normalization revealed similar trend.
Cells were probed using the neuronal marker βIII Tubulin (Abcam), and subsequently were incubated with the flourescein-conjugated goat anti-rabbit Alexa Flour 488 antibody (Invitrogen).
In parallel experiments, BrdU-treated slices were kept in culture and fixed at DIV30 and the OB was analyzed using the neuronal marker NeuN.
In agreement with the published reports, immunostaining using the neuronal marker NeuN reveal severe neurodegeneration in rTg4510 Tau mice (Fig 4A and B, WT vs. Tau).
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The present study was conducted to examine alterations of the intrinsic hepatic innervation at several time points following PH in rats, using the neuronal markers PGP9.5 and GAP-43.
We have used the neuronal markers, fluoro-Jade B (FJB) [ 28, 29] and NeuN [ 30], to examine the infected mouse brains.
To test whether and how the α7 nAChR-regulated NF-κB signaling is linked to the pathogenesis and senescence of HIV-1 gp41-I90, METH and C. neoformans invasion, we have used the neuronal markers, fluoro-Jade B (FJB) [ 28, 29] and NeuN [ 30], to examine the infected mouse brains.
Briefly, larvae were dissected in room temperature PBS and fixed for 30 minutes in 4% paraformaldehyde, immunostained at 4°C overnight using the neuronal membrane marker rabbit anti-HRP (1 100; Jackson ImmunoResearch, PA, 323-005-021) alone or with mAb 22C10 to label microtubules (mouse anti-Futsch, 1 50; Developmental Studies Hybridoma Bank, University of Iowa, Iowa City, IA).
GFAP levels were quantified in the frontal and parietal cortex of the same four cohorts of animals used for the neuronal marker determinations described in the previous section (Figure 6D, ANOVA p = 0.05 and ANOVA p = 0.0062, respectively).
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