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3D neuron models were reconstructed using the Neurolucida system (MBF Bioscience, USA) and a bright-field light microscope (Olympus, BX51, Japan).
Sections were analyzed with a Zeiss axioscope (Zeiss, Oberkochen, Germany) and photographs were made using the Neurolucida system (Microbrightfield, Magdeburg, Germany).
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All counting and measurements were performed using the Neurolucida software.
For some neurons, three-dimensional reconstructions were made using the Neurolucida software (Microbrightfield, Inc, USA).
To determine the maturation of neurons differentiated from transplanted SENAs, the longest neurite length per cell was measured using the Neurolucida software (MicroBrightField Europe).
For each experimental condition, a sample of PC, ranging from 40 to 90 cells, with no or minimal contact with other cells, were randomly selected from 3 4 different experiments and their axons carefully reproduced and measured using the Neurolucida software.
The number of BrdU-positive cells in a square area (2500 µm2) was counted on at least four serial sections from the midbrain of stage-matched littermates (n = 4) using the Neurolucida 6 software (MBF Bioscience, Williston, VT/USA).
The morphometric Sholl analysis was obtained from reconstructed neurons using the Neurolucida and NeuroExplorer software (MicroBrightField, Williston, USA).
Maximum intensity projections of the PV/WFA staining were analyzed by manual counting using the Neurolucida software.
Quantitative data relating to dendritic parameters for each reconstructed neuron were obtained using the Neurolucida Explorer software.
Representative examples were reconstructed using the Neurolucida software (MicroBrightfield Europe, Magdeburg, Germany) equipped to an Olympus BX61 microscope (Olympus, Hamburg, Germany).
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