Sentence examples for using the neomycin selection from inspiring English sources
Immortalized MEFs Esco2fl/ Δ transfected with the constructs were selected for stable integration using the neomycin selection and FACS sorting.
CHO cells were transfected with pIRES-hGLP1R by lipofection and selected using the neomycin resistance gene on the pIRES vector.
In theory, an effective alternative is drug resistance based selection of cardiomyocytes as successfully implemented by Field and collaborators using the Neomycin analogue G418 to purify cardiomyocytes from differentiating mouse ESC cultures [14].
Genotyping of the FltpZV mouse line (after Cre-mediated excision of the neomycin selection cassette) was performed using the forward primer 566 and 418 as well as the reverse primer 565.
The neomycin selection gene of pcDNA3 was subsequently replaced with Zeocin using NotI/MluI.
Mice lacking Arf (a gift of N. Sharpless) were generated with Cre-mediated excision of the neomycin selection cassette, similar to Ink4a mice [3].
LoxP sequences flanking the neomycin selection cassette are marked by triangles.
The full-length TP53 expression construct used for transfection of H1299 cells was constructed by recloning HindIII-NotI fragment from pSTARTA-TP53 into pcDNA3 in order to take advantage of the neomycin selection cassette in H1299 cells.
The neomycin selection cassette was removed by crosses of heterozygous p110β-RBD-DM mice with FLPe deleter mice, leaving a single FRT site in intron 6/7, which was used for PCR genotyping.
Deletion of the neomycin selection cassette by PhiC31 was verified by PCR for gene products specific for the recombined neomycin selection cassette (excised NEO), the unrecombined neomycin selection cassette (NEO), the STOP cassette, the wild-type Col1a1 locus and the mutant PhiC31 allele (Fig. 3D).
Successful PhiC31-mediated excision of the neomycin selection cassette was verified by PCR for gene products specific to the recombined neomycin selection cassette (excised NEO), the unrecombined neomycin selection cassette (NEO), the wild-type Col1a1 locus and the mutant PhiC31 allele (Fig. 3B).
