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The spacer sequences were compiled and aligned by using the multisequence alignment program ClustalX (1.8).
Sequencing products were purified and underwent electrophoresis with the 3100 Genetic Analyzer (Applied Biosystems) and aligned by using the multisequence alignment CLUSTALX version 1.8 (13 ).
The sequences were compared in the GenBank database (www.ncbi.nlm.nih.gov/GenBank) using the multisequence alignment Clustal within the BISANCE environment.
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Upstream and intronic genomic sequences for human and mouse Mef2c were obtained from both the Ensembl and UCSC Genome Browser web sites and were aligned using the multisequence local alignment tool MULAN (http://mulan.dcode.org/).org/
Sequences were aligned using the MUSCLE multisequence alignment program.
Sequence alignments were performed using the ClustalW multisequence alignment program, available on-line from the EMBL-EBI (http://www.ebi.ac.uk/clustalw/) [34].
The multisequence alignment was used to enrich annotation information and control the annotation quality.
For each column of the multisequence alignment, a codon score S was evaluated.
As an alternative to the multisequence alignment, the SHV variants are visualized as mutations relative to the sequence of SHV-1.
As an alternative to the multisequence alignment, the TEM variants are visualized as mutations relative to the sequence of TEM-1.
For each protein sequence, the DNA sequence was retrieved and codons were assigned to the respective amino acid in the multisequence alignment.
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