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The percent sequence identity with the C. crescentus XylD is presented for the four XylD homologs used and was obtained by aligning the protein sequences using the multiple sequence alignments tool Clustal Omega developed at the European Bioinformatics Institute (EMBL-EBI) (McWilliam et al. 2013).
These sequences were aligned with Clustal X 2.0 [42] by using the multiple sequence alignment.
HHpred submissions were also made using the multiple sequence alignments as queries.
The alignments were then shaded using the multiple sequence alignment editor GENEDOC http://www.nrbsc.org/gfx/genedoc/index.html.html
The concatenated protein sequences were aligned using the multiple sequence alignment tools in CLUSTAL X version 1.81 with the default settings [58].
Using the multiple sequence alignment technique, we have observed the similarity in the sequences and their respective alignment scores were elucidated.
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Using the multiple sequences alignment, we have generated scores on amino-acid matches and mismatches.
To assess the evolutionary relationships of the SlybHLH genes, an NJ phylogenetic tree was generated using the multiple sequences alignments of the conserved bHLH TF domains in tomato and Arabidopsis with a bootstrap value of 1,000.
If a user disagrees with this choice, they can rerun Pagan using an alternative strategy or use the multiple sequence alignment as input to Pplacer.
We used the multiple sequence alignment to generate a protein secondary structure prediction for the LNX3H domain on the Jpred 3 server [ 39].
We used the multiple sequence alignment methods (CLUSTALW) of the DNASTAR suite program MegAlign (Lasergene, v7.2; DNASTAR, Madison, Wisconsin, USA) (data not shown).
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