Exact(2)
To improve running times, the database can be filtered using the most intense peaks and user-defined constraints [98].
The Scherrer equation was used to determine the size of the crystallites using the most intense peak in all cases, which appears at approximate 32.79°.
Similar(58)
Thanks to the innovative configuration and the versatility shown by the dual cell linear ion trap MS used, the most intense and reliable peptide markers were first identified by untargeted survey experiment, and subsequently employed to design an ad hoc multi-target SRM method, based on the most intense transitions recorded for each selected precursor peptide.
For calculation C20-ganglioside percentage (Figure 5), we used the most intense ion peak derived from GM1 and GD1, namely [GM1-H] − and [GD1+K-2H]−, and intensities of these peaks in the summed spectra from each hippocampal region (at least 1300 spectra were summed for one region) were used for the calculation.
For each peptide we used the most intense seven transitions (y- and b-series ions) for initial measurement by unscheduled SRM.
The generated peptides were sequenced by nano-LC-tandem mass spectrometry (MS/MS) (Q-TOF Ultima with a nano-electrospray ionization source, Waters/Micromass, UK) in data-dependent acquisition (DDA) mode using the five most intense parent ions.
Raw spectra covering elution of the four main TTR isoforms were summarized and subsequently deconvoluted (i.e. decharged and deisotoped) by the Xtract algorithm within Qual Browser (Xcalibur ver. 2.0.7, Thermo Scientific) using the three most intense charge envelopes as described earlier [ 20].
Samples were analyzed with a 60-min linear gradient (5 35% acetonitrile with 0.2% formic acid) and data were acquired in a data-dependent manner, in which MS/MS fragmentation was performed using the six most intense peaks of every full MS scan.
To reach this goal the experiment uses one of the most intense continuous surface muon beams available (≈108μ/s) and relies on advanced technology (LXe calorimetry, a gradient-field superconducting spectrometer as well as flexible and powerful trigger and acquisition systems).
MALDI-MS was used to identify the most intense protein spots on the gel corresponding to proteins that were modulated by linoleic acid, and the identities of 38 up-regulated and 5 down-regulated proteins were unambiguously determined (Table 9 and 10).
For this purpose, (W_{{{text{Cu}}_{ 2} {text{O}}}} /W_{text{Cu}}) is considered as the ratio of weight percentage of copper oxide into that of copper, and by using the intensity of the most intense peaks of these two phases, which are located at (36.52^circ) (the most severe peaks of copper oxide) and (43.57^circ) (the most intense peaks of copper), we calculate the ratio.
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