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Quantification of cell growth on the titanium mesh material for a period of 28 days was accomplished using the molecular light imaging system.
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Gels were stained with ethidium bromide, viewed under UV light, and analyzed by using the Molecular Analyst System (Bio-Rad Laboratories).
In order to shed light on the biological significance of the FEM modules, we performed GSEA using the Molecular Signatures Database (MSigDB) [ 16].
qRT-PCR was performed using a Roche Molecular Light Cycler (Roche Molecular Biochemicals, Indianapolis, IN, USA).
Therefore, the crystal structure of MEPL was determined by molecular replacement using the bovine enteropeptidase light chain (PDB entry 1EKB, 53.7% amino acid identity) as the search model and refined to 2.0 Å resolution.
The molecular weight and the second virial coefficient of a protein sample in a solution can be measured using the static light scattering (SLS) technique.
It is important pointing out that the change in the value range in these experiments when compared with previous published work using the Light Cycler apparatus from Roche Molecular Biochemicals, is due to the higher sensitivity of the real-time PCR methodology performed here.
Absolute values of number-average (Mn) and weight-average (Mw) molecular weights were determined using the laser-light scattering (LLS) detection system.
ATP was measured using the ATP Determination Kit (Molecular Probes) based on luciferase's absolute requirement for ATP in producing light (emission maximum ~560 nm at pH 7.8).
Using the lighter, activate the paraffin cubes.
Always mix using the lighter color first.
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