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Nanometer-scale-thick, polymer-like coatings deposited using the molecular layer deposition (MLD) technique constitute a new class of materials.
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After charring, thin layer chromatography plates were scanned and analyzed using the Molecular Dynamic Personal Densitometer SI Scanner control software (GE Healthcare, Munich, Germany).
Ellipsometry data were analyzed using a two-layer model composed of a bulk gold layer underneath an organic layer, which was used to represent the molecular layer.
Several eGFP-positive cells were visualized at the hilus-granule cell layer border, in the granule cell layer and in the molecular layer using epifluorescence illumination (Fig. 1A).
The presynaptic density in granule cell axons in the molecular layer calculated using our method is higher than in previous estimates [60].
Whereas conventional methods identified two layers, the most external one corresponding to the molecular layer, by using immunohistochemical labelling for neurofilaments, three zones were distinguished.
ATR-FTIR, light microscopy, atomic force microscopy (AFM) and alternating current (ac) impedance spectroscopy have been used to characterize the molecular layers.
The molecular layer thickness was determined using Nikon EZ-C1 confocal microscope software.
The thicknesses of the molecular layer and the granule layer were measured on anterior and posterior portions of vermal sections of the cerebellum at 150-µm intervals using ImageJ National Institutes of Healthh, Bethesda, MD).
Their dendrites radiated into the molecular layer (Fig. 2B).
In the cerebellum CK1δ stained the molecular layer and the Purkinje cell layer (Figure 7J, K).
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