The SH-test [ 28] indicated that, using the molecular dataset, both the morphological tree and a tree in which each microhabitat specialization was constrained to be monophyletic were significantly less likely (all differences in likelihood exceeded 637 and all P < 0.001).
The independent test dataset accuracy rate denotes the percentage of samples in the test dataset that have been correctly diagnosed using the molecular signatures identified from classification of the training dataset.
Maximum likelihood (ML) tree of AGOs showing the approximate relative time of divergence of all the nodes using the molecular clock test on 'plant AGO dataset II'.
To elucidate the phylogenetic positions of two mysterious Asian genera, Gastrophrynoides and Phrynella, and to better understand the trans-continental distributions of microhylid taxa, we performed molecular phylogenetic and dating analyses using the largest molecular dataset applied to these taxa to date.
We also reviewed the phylogenetic relationships of afrobatrachians using the largest molecular dataset yet applied to this group.
Phylogenetic studies are undergoing a massive jump in the scale of the molecular datasets used to estimate phylogenies, due to the ease of collecting hundreds of loci sampled throughout the genomes of non-model taxa (e.g., [ 1, 2]).
The molecular dataset presented could be used in a comparative framework for future sampling, from both additional invaded and native sources.
Such networks rely on methods from graph theory that have recently been adapted to address an increasing number of biological questions using large molecular datasets [ 30, 31, 38- 43].
The present study addressed this issue using the hitherto longest molecular dataset (9,386 bp).
The data used here, the longest molecular datasets so far applied to afrobatrachian phylogeny, support the Afrobatrachia + Microhylidae hypothesis.
