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Cryogenic high-vacuum transfer into and out of the FIB/SEM was performed via a Quorum PT2010 cryo-preparation station using the modified specimen transfer shuttle device shown in Fig. 1e.
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The new procedure used in this study has been verified by the measuring of fracture toughness of Al alloy, using both the modified specimen, as designed here, and the standard one.
Fibrosis staging of each surgical resection specimen using the modified Ishak method was performed by a single liver pathologist.
Failure predictions of notched specimens using the modified approach result in significantly reduced conservatism compared to the standard Failure Assessment Diagram assessment method.
Genomic DNA was extracted from fruiting bodies dried in silica gel or from herbarium specimens using the modified CTAB method [ 35].
Sputum specimens were processed using the modified Petroff's method and concentrated by centrifugation at 3500 g in a refrigerated (4°C) centrifuge for 15 minutes [ 13].
Genomic DNA of each individual was extracted from silica gel dried leaves using the modified CTAB method (Doyle and Doyle, 1987), and one voucher specimen per population was preserved in the IBSC herbarium (Huiyu-DA1, Huiyu-MH1, Huiyu-WN1, and Huiyu-YC1).
The study participants were asked to provide weekly fecal specimens for microscopic detection of ova and parasites, including Cryptosporidium spp. Stool specimens were washed and concentrated by using the modified Ritchie formalin-ether method and examined for Cryptosporidium spp. oocysts by microscopy of smears stained with a modified acid-fast stain.
Using the modified key of Montecchi and Sarasini [16], clusters 4 and 18 are taxonomically uniform, including only specimens identified as H. griseus.
This study focused on HCC with a single aetiology, chronic HBV infection, and used surgical resection specimens to accurately score the severity of fibrosis and inflammation in the non-neoplatic liver using the modified Ishak method and HAI.
The wear and corrosion behavior of the modified specimens was studied respectively using pin-on-disc tribometer and anodic polarization tests.
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